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1A. Add profiles to your exoglycosidase panel.

If you applied a New England Biolab exoglycosidase to your profile select it.

  • A main profile must be provided - this is the profile where exoglycosidases will be applied to.
  • The specificities and order of the exoglycosidases in the list are important

1B. Paste the UPLC-MS profiles as a list.

In the text areas below you can enter UPLC-MS profiles (GU, % Area, Mass, and Charge) for each exoglycosidase used.
Tip: Mass and charge can be set to 'not available; values with the 'NA' token. Also quantitation is not required - % area can be set to anything above 0%.
(Click here to paste an example)


MAIN profile

NAN1 profile

ABS profile

BKF profile

AMF profile

BTG profile

GUH profile

CBG profile

SPG profile

JBM profile

Generate input file

1A. Add profiles to your exoglycosidase panel.

If you applied a New England Biolab exoglycosidase to your profile select it.

  • A main profile must be provided - this is the profile where exoglycosidases will be applied to.
  • The specificities and order of the exoglycosidases in the list are important

1B. Paste the mass (MS1) and peak list.

In the text areas below you can enter the MS signal (retention time, m/z and intensity) for the entire chromatogram and all the peaks (retention time, area and GU).
(Click here to paste an example)


MAIN profile

NAN1 profile

ABS profile

BKF profile

AMF profile

BTG profile

GUH profile

CBG profile

CBG profile

SPG profile

JBM profile

Merge data to input file

1. Upload exoglycosidase file

Upload a file containing Glucose Units (GUs) and peak areas. Mass and charge columns will increase assignment accuracy.

Generate input file by supplying peaks and mass or merging 3D mass and peak information.

Upload file    

2. Select a peak to analyze

Select the peak that you want to determine glycan structures in the main profile.

3. Select a glycoprotein (optional)

Supplying glycoprotein information will limit glycan structural variability and thus increase the accuracy.

Ranked list of N-glycans for

Below are the N-glycan ratings, scored by experimentally determined mass changes and GU shifts.
Remove glycans, as needed (e.g. α-galactose in human), and assign them to the chromatogram.

Peak analysis status:
0 of peaks (0%).

Keep? Score Oxford
notation		 Diagram Details More
information		 Graphical analysis ID

Digestions have no effect on mass and GU but peak area increases or remains the same. Below are the N-glycan ratings, scored only by delta GU.
Remove glycans, as needed (e.g. α-galactose in human), and assign them to the chromatogram.

Peak analysis status:
0 of peaks (0%).

Keep? Score Oxford
notation		 Diagram Details More
information		 Graphical analysis ID

Chromatogram Summary

This page summarizes the currently assigned N-glyan peaks.

Score threshold:
    Include:
       

Peak analysis status:
0 of peaks (0%).
N-glcyan abundance accounted for: 0 %.